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KMID : 0356619870020010035
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Journal of Korean Society of Endocrinology
1987 Volume.2 No. 1 p.35 ~ p.39
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Determination of Nuclear Progesterone Receptor in Rat Uterus
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Abstract
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The purpose of this study was to establish optimal conditions to measure for nuclear progesterone receptor, thereby applying this system to correlate pattern of receptor with a biological response of progesterone in physiological and pathological conditions. the assay was performed at 0℃ with ^(3)H-R5020, whch has 5 times greater affinity than that of endogenous progesterone as a radioligand using rat uterus.
The results obtained are as follow;
1) Specifis binding reached maximum after 2 hrs incubtion and were gradually decreased thereafter.
2) Nuclear receptors were saturated with 12nM ^(3)H-R5020 and concentration of 0.15~0.30mg DNA was used throughout the experiment.
3) Binding of ^(3)H-R5020 to nuclear receptor was displaced by the addition of R5020 and progesterone but not by the addition of cortisol and moderate dose of estradiol.
4) Polypropylene tube as an incubation tubr contributed to the high non-specific binging but poly styrene or siliconized glass tubes decreased non-specific binding.
5) By Scatchard analysis, Kd value for nuclear progesterone receptor was 3.07 nM.
6) Assay validity was evaluated by the coefficient variance (CV); CV for intraassay was 3.40% and CV for interassay 7.68%.
It is, therefore, considered that this assay system might be adequate to measure nuclear progesterone receptor for the clinical purpose as well as research purpose.
Rifampin is an inducer of hepatic drug metabolizng enzymes. This result in interaction with several drugs metabolized through the liver which is known to be the major organ responsible for steroid inactivation.
Thus concurrent treatment of rifampin and glucocorticoid hormone can lead to decreased bioavailability of glucocorticoid hormone and to increased requirement.
We report a case of rifampin-induced adrenal crisis who has received a physiologic maintenance dose of prednisolon and rifampicin.
It is terfore recommended that the steroid replacement needs to be adjusted according to the clinical response and serum electrolyte concentrations. This may require three to fourfold increment of the daily dosage of steroids.
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